With the development of the chemical industry and the development and utilization of natural compounds, the global environmental pollution has become more and more serious. According to relevant news reports, more than 350 varieties have been identified as environmental pollutants. People can find these pollutants in the air, in the soil, in the water, in fish and in poultry, and some cannot be degraded. At the same time, these pollutants pass through the food chain, which will further contaminate meat, eggs, food, vegetables, fruits and so on. The detection of environmental pollution became one of the important research topics in analytical chemistry as early as the beginning of the last century.
High performance liquid chromatography (HPLC) is a new separation analysis technology developed on the basis of classical liquid chromatography and gas chromatography. It uses a new high-pressure infusion pump, high-sensitivity detector and high-efficiency particle stationary phase and various intelligent software. It has higher efficiency, higher separation speed and faster speed than classical liquid chromatography.
In recent years, high performance liquid chromatography technology has developed rapidly, especially in environmental monitoring. In developed countries, high performance liquid chromatography (HPLC) is used as a common environmental monitoring method, such as the US EPA 531 method, using a high performance liquid chromatograph to configure a fluorescence detector to determine N-methyl carbamate pesticides in drinking water; EPA 547 method for the determination of glyphosate in drinking water by high performance liquid chromatography / fluorescence method; EPA 550 method for the determination of polycyclic aromatic hydrocarbons in drinking water by high performance liquid chromatography / UV and fluorescence; EPA605 method is used in high performance liquid chromatography Determination of benzidines in wastewater by electrochemical method; determination of polycyclic aromatic hydrocarbons in wastewater by high performance liquid chromatography/UV and fluorescence method using EPA610 method; determination of amino groups in wastewater by high performance liquid chromatography with post-column derivatization fluorimetry in EPA6610 method Formate pesticides; EPA6651 method for the determination of glyphosate herbicides in wastewater by high performance liquid chromatography; EPA 8310 method for the analysis of polycyclic aromatic hydrocarbons in solid waste by LC/fluorescence, even for harmful organic compounds in gases Determination by high performance liquid chromatography.
High performance liquid chromatography has been widely used in environmental monitoring, especially for the separation and analysis of organic pollutants with large molecular weight, low volatility and poor thermal stability. Such as polycyclic aromatic hydrocarbons, phenols, polycyclic biphenyls, phthalates, benzidines, anionic surfactants, organic pesticides, herbicides and the like.
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