Based on the core technology of independent research and development of scientific instruments, Shanghai Xinzhuang Instrument has not only developed low-temperature constant temperature instruments, ultrasonic instruments, box-type instruments, microbial instruments, ice-making refrigeration instruments, laboratory supporting instruments, but also gradually formed various A scientific product technology product reserve product product development pattern. The solid phase extraction technology of the LNB brand solid phase extraction device developed by Shanghai Xinzhuang Instrument Co., Ltd. - since the advent of the solid phase extraction method in the late 1970s, the development of very fast SPE columns with different stationary phases has emerged. The solid phase extraction method is continuously adapted to the pretreatment requirements of various samples, and the solid phase extraction method for different kinds of compounds is gradually improved.
Solid Phase Extraction is the use of a solid adsorbent to adsorb a target compound in a liquid sample, separate it from the matrix and interfering compound of the sample, and then elute with an eluent or desorb to heat to achieve separation and enrichment. The purpose of the compound. As a sample preparation technology, solid phase extraction has been widely used in the laboratory.
SPE is a column chromatography process with many similarities to high performance liquid chromatography (HPLC) in terms of separation mechanism, choice of stationary phase and solvent. However, the particle size of the SPE column (>40!m) is larger than the HPLC packing (3~10!m). Due to the short column bed and large particle size, the SPE efficiency is much lower than for HPLC columns. A high-performance HPLC column can produce more than 10,000 plates, while a single SPE column can get 10 to 50 plates. Therefore, compounds with very different properties can only be separated by SPE. In this case, instead of isocratic, a typical SPE separation is performed in a digital switching manner (digitaIon-off mode). This means that, through a step gradient, the analyte is either not strongly adsorbed by the stationary phase or completely unretained. Another difference from HPLC is that the SPE column is disposable.
The characteristics of the SPE column itself determine the scope of application. SPE technology with low separation efficiency is mainly used to treat samples. The goal to be achieved with SPE is to:
(1) Removal from the sample for interference with subsequent analysis
(2) Enriching trace components to improve analytical sensitivity;
(3) changing the solvent of the sample to match the analytical method;
(4) in situ derivatization;
(5) Desalting the sample;
(6) Facilitate the storage and transportation of samples.
Among the above points, purification and enrichment are the most important.
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